Rapid and label-free bacteria detection by surface plasmon resonance (SPR) biosensors
author: Miss Fahriye C Dudak ceyda@hacettepe.edu.tr Ankara Turkey; Prof. Ismail Hakki Boyaci ihb@hacettepe.edu.tr Hacettepe University Department of Food Engineering Turkey
language: en

Abstract

Surface Plasmon Resonance (SPR) biosensor technology has been successfully used for the detection of various analytes such as proteins, drugs, DNA and microorganisms. SPR based immunosensors that coupled with specific antigen-antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid and label-free detection. In this paper, we review the important issues in the development of SPR-based immunoassays for bacteria detection, concentrating on instrumentation, surface functionalization, liquid hadling and surface regeneration. In addition, this review touches on the recent advances in SPR biosensing for sensitivity enhancement.