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A digital atlas of ion channel expression patterns in the two-week-old rat brain.
Shcherbatyy, V.
Carson, J.
Yaylaoglu, M.
Jäckle, K.
Grabbe, F.
Brockmeyer, M.
Yavuz, H.
Eichele, G.
The approximately 350 ion channels encoded by the mammalian genome are a main pillar of the nervous system. We have determined the expression pattern of 320 channels in the two-week-old (P14) rat brain by means of non-radioactive robotic in situ hybridization. Optimized methods were developed and implemented to generate stringently coronal brain sections. The use of standardized methods permits a direct comparison of expression patterns across the entire ion channel expression pattern data set and facilitates recognizing ion channel co-expression. All expression data are made publically available at the Genepaint.org database. Inwardly rectifying potassium channels (Kir, encoded by the Kcnj genes) regulate a broad spectrum of physiological processes. Kcnj channel expression patterns generated in the present study were fitted with a deformable subdivision mesh atlas produced for the P14 rat brain. This co-registration, when combined with numerical quantification of expression strengths, allowed for semi-quantitative automated annotation of expression patterns as well as comparisons among and between Kcnj subfamilies. The expression patterns of Kcnj channel were also cross validated against previously published expression patterns of Kcnj channel genes.
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
2015-01-01
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Neuroinformatics 13 1
eng
Shcherbatyy
V.
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
escidoc:persistent34
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Carson
J.
Yaylaoglu
M.
Jäckle
K.
Grabbe
F.
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
escidoc:persistent34
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Brockmeyer
M.
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
escidoc:persistent34
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Yavuz
H.
Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society
escidoc:578595
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Eichele
G.
Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society
escidoc:persistent34
http://pubman.mpdl.mpg.de/cone/persons/resource/persons15030
A digital atlas of ion channel expression patterns in the two-week-old rat brain.
eng
10.1007/s12021-014-9247-0
2015-01-01
http://purl.org/eprint/status/PeerReviewed
Neuroinformatics
13
1
111
125
The approximately 350 ion channels encoded by the mammalian genome are a main pillar of the nervous system. We have determined the expression pattern of 320 channels in the two-week-old (P14) rat brain by means of non-radioactive robotic in situ hybridization. Optimized methods were developed and implemented to generate stringently coronal brain sections. The use of standardized methods permits a direct comparison of expression patterns across the entire ion channel expression pattern data set and facilitates recognizing ion channel co-expression. All expression data are made publically available at the Genepaint.org database. Inwardly rectifying potassium channels (Kir, encoded by the Kcnj genes) regulate a broad spectrum of physiological processes. Kcnj channel expression patterns generated in the present study were fitted with a deformable subdivision mesh atlas produced for the P14 rat brain. This co-registration, when combined with numerical quantification of expression strengths, allowed for semi-quantitative automated annotation of expression patterns as well as comparisons among and between Kcnj subfamilies. The expression patterns of Kcnj channel were also cross validated against previously published expression patterns of Kcnj channel genes.
Ion channels; Gene expression analysis; In situ hybridization; Genepaint.org database; Rat brain; Digital atlas; Subdivision mesh
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